Computerized Medical Imaging and Graphics
Volume 34, Issue 3 , Pages 179-184 , April 2010

Automatic identification and morphometry of optic nerve fibers in electron microscopy images

  • Ximei Zhao

      Affiliations

    • College of Information Engineering, Qingdao University, Qingdao, China
    • Department of Anatomy, Histology and Embryology, Fudan University, Shanghai, China
  • ,
  • Zhenkuan Pan

      Affiliations

    • College of Information Engineering, Qingdao University, Qingdao, China
  • ,
  • Jinyan Wu

      Affiliations

    • Department of Anatomy, Histology and Embryology, Fudan University, Shanghai, China
  • ,
  • Guomin Zhou

      Affiliations

    • Department of Anatomy, Histology and Embryology, Fudan University, Shanghai, China
    • Corresponding Author InformationCorresponding authors. Tel.: +86 10 67391685.
  • ,
  • Yanjun Zeng

      Affiliations

    • Biomechanical and Medical Information Institute, Beijing University of Technology, Beijing 100022, China
    • Corresponding Author InformationCorresponding authors. Tel.: +86 10 67391685.

Received 5 May 2009 ,Revised 18 July 2009 ,Accepted 31 August 2009.

References 

  1. Walsh C. Age-related fiber order in the ferret's optic nerve and optic chiasm. J Neurosci. 1986;6:1635–1642
  2. Cepurnaa WO, Kaytonb RJ, Johnsona EC, Morrisona JC. Age related optic nerve axonal loss in adult Brown Norway rats. Exp Eye Res. 2005;80:877–884
  3. Saito HA. Morphology of physiologically identified X-, Y-, and W-type retinal ganglion cells of the cat. J Comp Neurol. 1983;221:279–288
  4. Stone J, Campion . Estimate of the number of myelinated axons in the cat's optic nerve. J Comp Neurol. 1978;180:799–806
  5. Wakakuwa K, Watanabe M, Sugimoto T, Washida A, Fukuda Y. An electron microscopic analysis of the optic nerve of the eastern chipmunk (Tamias sibiricus asiaticus): total fiber count and retinotopic organisation. Vis Res. 1987;27:1891–1901
  6. Cull G, Ag Sci B. Estimating normal optic nerve axon numbers in non-human. J Glaucoma. 2003;12:301–306
  7. Williams RW, Chalupa LM. An analysis of axon caliber within the optic nerve of the cat: evidence of size groupings and regional organization. J Neurosci. 1983;3(8):1554–1564
  8. Brooks DE, Strubbe DT. Histomorphometry of the optic nerves of normal dogs and dogs with hereditary glaucoma. Exp Eye Res. 1995;60:71–89
  9. Sanchez RM, Dunkelberger GR, Quigley HA. The number and diameter distribution of axons in the monkey optic nerve. Invest Ophthalmol Vis Sci. 1986;27:1342–1350
  10. Morrison JC, Moore CG, Deppmeier LM, Gold BG, Meshul CK, Johnson EC. A rat model of chronic pressure-induced optic nerve damage. Exp Eye Res. 1997;64:85–96
  11. Vita G, Santoro M, Trombetta G, Leonardi L, Messina C. A computer-assisted automatic method for myelinated nerve fiber morphometry. Acta Neurol Scand. 1992;85:18–22
  12. Fok YL, Chan JCK, Chin RT. Automated analysis of nerve-cell images automated analysis of nerve-cell images. IEEE Trans Med Imaging. 1996;15(3):353–368
  13. Campadelli P, Gangai C, Pasquale F. Automated morphometric analysis in peripheral neuropathies. Comp Biol Med. 1999;29:147–156
  14. Silva APD, Jordão CER, Fazan VPS. Peripheral nerve morphometry: comparison between manual and semi-automated methods in the analysis of a small nerve. J Neurosci Methods. 2007;159:153–157
  15. Klemenĉiĉ Aleš, Kovaĉiĉ Stanislav. Automated segmentation of muscle fiber images using active contour models. Cytometry. 1998;32:317–326
  16. Romero E, Cuisenaire O. Automatic morphometry of nerve histological sections. J Neurosci Methods. 2000;97:111–122
  17. Otsu N. A threshold selection method from gray level histograms. IEEE Trans Syst Man Cyber. 1979;9:62–66
  18. Sonka M, Hlavac V, Boyle R. Image processing, analysis, and machine vision. second ed.. Thomson Learning and PPTPH; 2002;pp. 232–235
  19. Drenhaus U, Gunten AV, Rager G. Classes of axons and their distribution in the optic nerve of the tree shrew (Tupaia belangeri). Anat Rec. 1997;249:103–116

PII: S0895-6111(09)00111-6

doi: 10.1016/j.compmedimag.2009.08.009

Computerized Medical Imaging and Graphics
Volume 34, Issue 3 , Pages 179-184 , April 2010